Delafield's Hematoxylin TS
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This article is compiled based on the United States Pharmacopeia (USP) – 2025 Edition
Issued and maintained by the United States Pharmacopeial Convention (USP)
-Prepare 400 mL of a saturated solution of ammonium alum (Solution A). Dissolve 4 g of hematoxylin in 25 mL of alcohol, mix it with Solution A, and allow it to stand for 4 days in a flask closed with a pledget of purified cotton and exposed to light and air (Solution B). Then filter Solution B, and add to it a Solution C consisting of a mixture of 100 mL of Glycerin and 100 mL of methanol. Mix, and allow the mixture to stand in a warm place, exposed to light, for 6 weeks until it becomes dark-colored. Store in tightly stoppered bottles.
For use in staining endocrine tissue, dilute this test solution with an equal volume of water.
