Uridine
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This article is compiled based on the United States Pharmacopeia (USP) – 2025 Edition
Issued and maintained by the United States Pharmacopeial Convention (USP)
C9H12N2O6 244.20 CAS RN®: 58-96-8.—White powder.
Assay
Mobile phase: Prepare a mixture of methanol and 0.2 M ammonium acetate (10:90), and adjust with phosphoric acid to a pH of 7.0.
Test solution: 0.5 mg per mL in water.
Procedure: Inject about 20 μL of the Test solution into a liquid chromatograph (see Chromatography 〈621〉), equipped with a 280-nm detector and a 4.6-mm × 15-cm column that contains packing L1. The flow rate is about 2.0 mL per minute. The area of the C H N O peak is not less than 99% of the total peak area.
Melting Range 〈741〉: between 166° and 171°.
