Triphenyltetrazolium Chloride
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This article is compiled based on the United States Pharmacopeia (USP) – 2025 Edition
Issued and maintained by the United States Pharmacopeial Convention (USP)
C19H15ClN4 334.80 CAS RN®: 298-96-4 -White to yellowish, crystalline powder. Soluble in about 10 parts of water and of alcohol; slightly soluble in acetone; insoluble in ether. Usually contains solvent of crystallization, and when dried at 105° it melts at about 240°, with decomposition.
Solubility: Separate 100-mg portions dissolve completely in 10 mL of water and in 10 mL of alcohol, respectively, to yield solutions that are clear, or practically so.
Loss on Drying 〈731〉: Dry it at 105° to constant weight: it loses not more than 5.0% of its weight.
Residue on Ignition (Reagent test): negligible, from 100 mg.
Sensitiveness: Dissolve 10 mg in 10 mL of dehydrated alcohol (A). Then dissolve 10 mg of dextrose in 20 mL of dehydrated alcohol (B). To 0.2 mL of B add 1 mL of dehydrated alcohol and 0.5 mL of dilute tetramethylammonium hydroxide TS (1 volume diluted with 9 volumes of dehydrated alcohol), then add 0.2 mL of A: a pronounced red color develops within about 10 minutes.

