Sudan IV
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This article is compiled based on the United States Pharmacopeia (USP) – 2025 Edition
Issued and maintained by the United States Pharmacopeial Convention (USP)
C24H20N4O 380.44 CAS RN®: 85-83-6.—Brown to reddish-brown powder.
Assay: Transfer about 25 mg, accurately weighed, to a 100-mL volumetric flask. Dissolve in chloroform, dilute with chloroform to volume, and mix. Dilute 2.0 mL of the resulting solution with chloroform to 50.0 mL. Determine the absorbance of this solution in 1-cm cells at the wavelength of maximum absorbance at about 520 nm, with a suitable spectrophotometer, using chloroform as the blank. Calculate the percentage of Sudan IV in the test specimen taken by the formula:
(100A)/(85C)
in which A is the absorbance at 520 nm and C is the concentration of the test specimen in g per L. Not less than 90% is found.
Loss on Drying〈731〉: Dry it at 105° for 2 hours: it loses not more than 10% of its weight.
