Potassium Hyaluronate
If you find any inaccurate information, please let us know by providing your feedback here
This article is compiled based on the United States Pharmacopeia (USP) – 2025 Edition
Issued and maintained by the United States Pharmacopeial Convention (USP)
White to cream-colored powder. Freely soluble in water. Store in a tight container, in a refrigerator.
Inhibitor Content: Prepare as directed in the Assay under Hyaluronidase for Injection (USP monograph) a quantity of Standard solution containing 1 USP Hyaluronidase Unit in each mL, and a similar quantity of acetate-buffered Standard solution using as the solvent 0.1 M, pH 6 sodium acetate buffer (prepared by diluting the 0.2 M buffer prepared as directed below with an equal volume of water). Prepare from the potassium hyaluronate under test 10 mL of Potassium hyaluronate stock solution, and dilute 2 mL of it with the specified Phosphate buffer solution to make a Hyaluronate solution. In the same way, and concurrently, dilute a second 2-mL portion of the stock solution with 0.2 M, pH 6 sodium acetate buffer (containing 16.4 g of anhydrous sodium acetate and 0.45 mL of glacial acetic acid in each 1000 mL).
Place 0.50-mL portions of the Hyaluronate solution in each of four 16- × 100-mm test tubes, and place 0.50-mL portions of the acetate-buffered
Hyaluronate solution in two similar tubes. To two of the four tubes containing Hyaluronate solution add 0.50 mL of Diluent for hyaluronidase solutions, prepared as directed in the Assay under Hyaluronidase for Injection (USP monograph). To the remaining two tubes, on a rigid schedule, at 30-second intervals, add 0.50 mL of Standard solution. Similarly, to the two tubes containing acetate-buffered Hyaluronate solution add at 30-second intervals 0.50-mL portions of acetate-buffered Standard solution. Then proceed as directed in the second paragraph for Procedure, beginning with “Mix the contents,” as far as “Plot the average.” The reduction in absorbance of acetate-buffered Hyaluronate solution is not less than 25% of that observed in the Hyaluronate solution.
Turbidity production: The average absorbance of the solutions in the two tubes containing Hyaluronate solution and Diluent for hyaluronidase solutions prepared in the test for Inhibitor Content is not less than 0.26 at a wavelength of 640 nm in a suitable spectrophotometer using a 1-cm cell.
