pH 7.4 Phosphate Buffered Saline TS
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This article is compiled based on the United States Pharmacopeia (USP) – 2025 Edition
Issued and maintained by the United States Pharmacopeial Convention (USP)
—Transfer 80 g of sodium chloride, 2.0 g of potassium chloride, 14.4 g of anhydrous dibasic sodium phosphate, and 2.4 g of monobasic potassium phosphate to a 1-L volumetric ask containing about 800 mL of water. Mix until completely dissolved. Adjust with diluted hydrochloric acid to a pH of 7.4. Dilute with water to volume. Sterilize by autoclaving.
[Note—Commercially available pH 7.4 phosphate buffered saline solutions can be used.]
(USP 1-Dec-2021)

