p-Iodonitrotetrazolium Violet
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This article is compiled based on the United States Pharmacopeia (USP) – 2025 Edition
Issued and maintained by the United States Pharmacopeial Convention (USP)
(2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride), C19H13ClIN5O2 505.70-Light yellow powder.
ASSAY: When tested by thin-layer chromatography, with the use of plates coated with chromatographic silica gel mixture and a developing system consisting of a mixture of amyl alcohol, formic acid, and water (8:1:1), sprayed with 0.1% sodium thiosulfate solution, and examined under short-wavelength UV light, a single spot is exhibited, with trace impurities.
MELTING POINT (741): 240°, with decomposition.
