Neomycin Sulfate and Bacitracin Ointment

This article is compiled based on the United States Pharmacopeia (USP) – 2025 Edition

Issued and maintained by the United States Pharmacopeial Convention (USP)

DOWNLOAD PDF HERE

Neomycin Sulfate and Bacitracin Ointment contains the equivalent of not less than 90.0 percent and not more than 130.0 percent of the labeled amounts of neomycin and bacitracin.

1 Packaging and storage

Preserve in tight, light-resistant containers, preferably at controlled room temperature.

1.1 USP Reference standards 〈11〉

USP Bacitracin Zinc RS

USP Neomycin Sulfate RS

Thin-layer chromatographic identification test 〈201BNP〉: meets the requirements.

Minimum fill 〈755〉: meets the requirements.

Water Determination, Method I 〈921〉: not more than 0.5%, 20 mL of a mixture of toluene and methanol (7:3) being used in place of methanol in the titration vessel.

2 Assay for neomycin

Proceed with Ointment as directed in the Assay under Neomycin Sulfate Ointment.

3 Assay for bacitracin

Proceed with Ointment as directed in the Assay under Bacitracin Ointment.