Neomycin and Polymyxin B Sulfates and Hydrocortisone Acetate Cream

This article is compiled based on the United States Pharmacopeia (USP) – 2025 Edition

Issued and maintained by the United States Pharmacopeial Convention (USP)

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Neomycin and Polymyxin B Sulfates and Hydrocortisone Acetate Cream contains the equivalent of not less than 90.0 percent and not more than 130.0 percent of the labeled amounts of neomycin and polymyxin B, and not less than 90.0 percent and not more than 110.0 percent of the labeled amount of hydrocortisone acetate (C₂₃H₃₂O₆).

Packaging and storage-Preserve in well-closed containers.

USP REFERENCE STANDARDS (11)-

USP Hydrocortisone Acetate RS

USP Neomycin Sulfate RS

USP Polymyxin B Sulfate RS

Identification-

A: It meets the requirements under Thin-Layer Chromatographic Identification Test (201BNP).

B: The retention time of the major peak for hydrocortisone acetate in the chromatogram of the Assay preparation corresponds to that in the chromatogram of the Standard preparation, as obtained in the Assay for hydrocortisone acetate.

MINIMUM FILL (755): meets the requirements.

Assay for neomycin and Assay for polymyxin B-Proceed with Cream as directed in the Assay for neomycin and in the Assay for polymyxin B under Neomycin and Polymyxin B Sulfates Cream.

Assay for hydrocortisone acetate-Proceed with Cream as directed in the Assay under Hydrocortisone Acetate Lotion.