Hematoxylin
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This article is compiled based on the United States Pharmacopeia (USP) – 2025 Edition
Issued and maintained by the United States Pharmacopeial Convention (USP)
(Hydroxybrasilin), C16H14O6·3H2O 356.32 CAS RN®: 517-28-2.-A crystalline substance derived from the heartwood of Haematoxylon campechianum Linné (Fam. Leguminosae). Colorless to yellow prisms. Very slightly soluble in cold water and in ether; rapidly soluble in hot water and in hot alcohol. When exposed to light, it acquires a red color and yields a yellow solution. Dissolves in ammonia TS and in solutions of alkali hydroxides and carbonates. When dissolved in solutions of the following salts, it develops the colors indicated: in alum solution, a red color; in stannous chloride solution, a rose color; and in solutions of cupric salts, a greenish-gray color. It gradually turns black in potassium dichromate solution. Store hematoxylin and its solutions protected from light and air.
