Ethyl Vanillin

This article is compiled based on the United States Pharmacopeia (USP) – 2025 Edition

Issued and maintained by the United States Pharmacopeial Convention (USP)

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C₉H₁₀O₃ 166.17

Benzaldehyde, 3-ethoxy-4-hydroxy-;

3-Ethoxy-4-hydroxybenzaldehyde CAS RN®: 121-32-4.

1 DEFINITION

Ethyl Vanillin, dried over phosphorus pentoxide for 4 h, contains NLT 98.0% and NMT 101.0% of C₉H₁₀O₃

2 IDENTIFICATION

Change to read:

A. Spectroscopic Identification Tests 〈197〉, Infrared Spectroscopy: 197K (CN 1-May-2020)

Change to read:

B. Spectroscopic Identification Tests 〈197〉, Ultraviolet-Visible Spectroscopy: 197U (CN 1-May-2020)

Sample solution: 8 μg/mL in methanol

Acceptance criteria: Meets the requirements

3 ASSAY

Procedure

Sample: 300 mg of Ethyl Vanillin (previously dried)

Titrimetric system

(See Titrimetry 〈541〉.)

Mode: Direct titration

Titrant: 0.1 N sodium methoxide VS

Blank: 50 mL of dimethylformamide, accurately measured

Endpoint detection: Visual

Analysis: Transfer the Sample solution to a 125-mL conical flask, and dissolve in 50 mL of dimethylformamide. Add thymol blue TS and titrate, using a magnetic stirrer and taking precautions against the absorption of atmospheric carbon dioxide. Perform a blank determination.

Calculate the percentage of ethyl vanillin (C₉H₁₀O₃) in the Sample taken:

Result = {[(V_S − V_B) × N × F]/W} × 100

V_S = Titrant volume consumed by the Sample (mL)

V_B = Titrant volume consumed by the Blank (mL)

N = actual normality of the Titrant (mEq/mL)

F = equivalency factor, 166.2 mg/mEq

W = Sample weight (mg)

Acceptance criteria: 98.0%–101.0% on the previously dried basis

4 IMPURITIES

Residue on Ignition 〈281〉: NMT 0.1%

5 SPECIFIC TESTS

Melting Range or Temperature 〈741〉: 76°–78°

Loss on Drying 〈731〉: Dry a sample over phosphorus pentoxide for 4 h: it loses NMT 1.0% of its weight.

6 ADDITIONAL REQUIREMENTS

Packaging and Storage: Preserve in tight, light-resistant containers.

USP Reference Standards 〈11〉

USP Ethyl Vanillin RS