Crystal Violet

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Crystal Violet

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This article is compiled based on the United States Pharmacopeia (USP) – 2025 Edition

Issued and maintained by the United States Pharmacopeial Convention (USP)

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(Hexamethyl-p-rosaniline Chloride), C25H30ClN3 —407.98—Dark-green crystals. Slightly soluble in water; sparingly soluble in alcohol and in glacial acetic acid. Its solutions are deep violet in color.

Sensitiveness: Dissolve 100 mg in 100 mL of glacial acetic acid, and mix. Pipet 1 mL of the solution into a 100-mL volumetric flask, and dilute with glacial acetic acid to volume: the solution is violet-blue in color and does not show a reddish tint. Pipet 20 mL of the diluted solution into a beaker, and titrate with 0.1 N perchloric acid VS, adding the perchloric acid slowly from a microburet: not more than 0.10 mL of 0.1 N perchloric acid is required to produce an emerald-green color.

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