Benzyl Benzoate

This article is compiled based on the United States Pharmacopeia (USP) – 2025 Edition

Issued and maintained by the United States Pharmacopeial Convention (USP)

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C₁₄H₁₂O₂       212.24

Benzoic acid, phenylmethyl ester;

Benzyl benzoate CAS RN: 120-51-4.

1 DEFINITION

Benzyl Benzoate contains NLT 99.0% and NMT 100.5% of CHO 14 12 2

2 IDENTIFICATION

Change to read:

A. SPECTROSCOPIC IDENTIFICATION TESTS (197), Infrared Spectroscopy: 197E _{(CN 1-MAY-2020)}

3 ASSAY

PROCEDURE

Sample: 2 g of Benzyl Benzoate

Analysis: Transfer the Sample to a conical flask fitted with a reflux condenser. Add 50.0 mL of 0.5 N alcoholic potassium hydroxide VS, and boil gently for 1 h. Cool, add phenolphthalein TS, and titrate with 0.5 N hydrochloric acid VS. Perform a blank determination (see Titrimetry. (541)). Each mL of 0.5 N alcoholic potassium hydroxide is equivalent to 106.1 mg of Benzyl Benzoate (C₁₄H₁₂O₂).

Acceptance criteria: 99.0%-100.5%

4 IMPURITIES

LIMIT OF ALDEHYDES

Sample solution: Transfer 10.0 g to a 125-ml. conical flask containing 50 mL of alcohol and 5 mL of hydroxylamine hydrochloride solution (3.5 in 100), mix, and allow to stand for 10 min.

Analysis: Add 1 mL of bromophenol blue TS, and titrate with 0.1 N sodium hydroxide VS to a light green endpoint. Perform a blank determination, and match the color of the endpoint with that of the titrated Sample solution.

Acceptance criteria: The net volume of 0.1 N sodium hydroxide consumed does not exceed 0.50 mL (0.05% as benzaldehyde).

5 SPECIFIC TESTS

SPECIFIC GRAVITY (841): 1.116-1.120

CONGEALING TEMPERATURE (651): Congelation may be brought about by the addition of a fragment of previously congealed Benzyl Benzoate when the temperature has reached the expected congealing temperature.

Acceptance criteria: NLT 18.0°

REFRACTIVE INDEX (831): 1.568-1.570 at 20°

ACIDITY: Add 2 drops of phenolphthalein TS to 25 mL of alcohol, and add 0.020 N sodium hydroxide until a pink color is produced. Add 5.0 g of Benzyl Benzoate, and titrate with 0.020 N sodium hydroxide.

Acceptance criteria: NMT 1.5 mL of 0.020 N sodium hydroxide is required to restore the pink color.

6 ADDITIONAL REQUIREMENTS

PACKAGING AND STORAGE: Preserve in tight, well-filled, light-resistant containers, and avoid exposure to excessive heat.

USP REFERENCE STANDARDS (11)

USP Benzyl Benzoate RS