Acetylcholine Chloride
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This article is compiled based on the United States Pharmacopeia (USP) – 2025 Edition
Issued and maintained by the United States Pharmacopeial Convention (USP)
(Trimethylethanaminium Chloride; Acecoline), [CH3COOCH2CH2N(CH3)3]Cl 181.66 CAS RN®: 60-31-1.—White, crystalline powder. Very deliquescent; very soluble in water; freely soluble in alcohol.
Melting Range〈741〉: When previously dried at 110° in a capillary tube for 1 hour, it melts between 149° and 152°.
Reaction: A solution (1 in 10) is neutral to litmus.
Residue on Ignition (Reagent test): negligible, from 200 mg.
Solubility in Alcohol: A solution of 500 mg in 5 mL of alcohol is complete and colorless.
Percent of Acetyl (CH3CO): Weigh accurately about 400 mg, previously dried at 105° for 3 hours, and dissolve in 15 mL of water in a glass- stoppered conical flask. Add 40.0 mL of 0.1 N sodium hydroxide VS, and heat on a steam bath for 30 minutes. Insert the stopper, allow to cool, add phenolphthalein TS, and titrate the excess alkali with 0.1 N sulfuric acid VS. Determine the exact normality of the 0.1 N sodium hydroxide by titrating 40.0 mL after it has been treated in the same manner as in the test. Each mL of 0.1 N sodium hydroxide is equivalent to 4.305 mg of CH3CO. Between 23.2% and 24.2% is found.
Percent of Chlorine (Cl): Weigh accurately about 400 mg, previously dried at 105° for 3 hours, and dissolve in 50 mL of water in a glass- stoppered, 125-mL flask. Add with agitation 30.0 mL of 0.1 N silver nitrate VS, then add 5 mL of nitric acid and 5 mL of nitrobenzene, shake, add 2 mL of ferric ammonium sulfate TS, and titrate the excess silver nitrate with 0.1 N ammonium thiocyanate VS: each mL of 0.1 N silver nitrate is equivalent to 3.545 mg of Cl. Between 19.3% and 19.8% of Cl is found.
