3,3′-Diaminobenzidine Hydrochloride,
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This article is compiled based on the United States Pharmacopeia (USP) – 2025 Edition
Issued and maintained by the United States Pharmacopeial Convention (USP)
(NH2)2C6H3C6H3(NH2)2 · 4HCI 360.11 CAS RN®:7411-49-6.-White to yellowish-tan (occasionally purple), needle-shaped crystals. Soluble in water. Stable in organic solvents but unstable in aqueous solution at room temperature. Store aqueous solutions in a refrigerator.
Insoluble Matter: Dissolve 2 g in 100 mL of water, without heating, and filter immediately: the insoluble residue does not exceed 1 mg (0.05%).
Residue on Ignition (Reagent test): not more than 1 mg, from 2 g (0.05%).
Suitability Test for Detection of Selenium: Dissolve 1.633 g of selenious acid (H2SeO3) in water, and dilute with water to 1 L. Dilute 10 mL of this solution with water to 1 L, to make a solution containing 0.010 mg of Se per mL. Place 1 mL of the resulting solution in a 100-mL beaker, add 2 mL of formic acid solution (1 in 7), and dilute with water to 50 mL. Add 2 mL of 3,3'-diaminobenzidine hydrochloride solution (1 in 200), and allow to stand for 30 to 50 minutes. Adjust with 6 N ammonium hydroxide to a pH between 6 and 7. Transfer to a 125-mL separator, add 10.0 mL of toluene, and shake vigorously for 30 seconds: a distinct yellow color is produced in the toluene layer. A blank containing diaminobenzidine hydrochloride but no Selenium standard, treated in the same manner, shows no color in the toluene layer.
